Biochemical and Genetic Characterization of nirB Mutants of Escherichia coli K12 Pleiotropically Defective in Nitrite and Sulphite Reduction
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چکیده
منابع مشابه
Biochemical and genetic characterization of nirB mutants of Escherichia coli K 12 pleiotropically defective in nitrite and sulphite reduction.
Mutants of Escherichia coli K12 defective in the nirB gene lack NADH-dependent nitrite reductase activity and reduce nitrite slowly during anaerobic growth. With one exception these mutants require cysteine for growth. Cytochrome C552 synthesis and the assimilation of ammonia are unaffected by the nirB mutation. The defective gene is located between the crp and aroB genes at minute 73 on the E...
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Purification and properties of nitrite reductase from Escherichia coli K12.
NADH-nitrite oxidoreductase (EC 1.6.4) was purified to better than 95% homogeneity from batch cultures of Escherichia coli strain OR75Ch15, which is partially constitutive for nitrite reductase synthesis. Yields of purified enzyme were low, mainly because of a large loss of activity during chromatography on DEAE-cellulose. The quantitative separation of cytochrome c-552 from nitrite reductase a...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-120-2-475